GABA-A R gamma 2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-151

Novus Biologicals, part of Bio-Techne
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NB300-151
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Turkey, Zebrafish

Cited:

Human, Mouse, Rat, Fish - Danio rerio (Zebrafish), Turkey

Predicted:

Bovine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all GABA-A R gamma 2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH. Accession # P18508

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16807363). Human reactivity reported in scientific literature (PMID: 24480790). Turkey reactivity reported in scientific literature (PMID: 27055929). Zebrafish reactivity reported in scientific literature (PMID: 28285877).

Localization

Cell Membrane

Specificity

Specific for endogenous levels of the ~46 kDa gamma 2-subunit of the GABAA receptor.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-A R gamma 2 Antibody - Azide and BSA Free

Western Blot: GABA-A R gamma 2 AntibodyAzide and BSA Free [NB300-151]

Western Blot: GABA-A R gamma 2 AntibodyAzide and BSA Free [NB300-151]

Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.
Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody - Azide and BSA Free [NB300-151]

Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody - Azide and BSA Free [NB300-151]

Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.
Western Blot: GABA-A R gamma 2 AntibodyAzide and BSA Free [NB300-151]

Western Blot: GABA-A R gamma 2 AntibodyAzide and BSA Free [NB300-151]

Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.

Applications for GABA-A R gamma 2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:400

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24480790). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28285877).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Neat whole antisera

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R gamma 2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl(-) channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).

Long Name

gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide

Alternate Names

GABAAR gamma 2, GABAARg2, GABRG2

Entrez Gene IDs

2566 (Human); 14406 (Mouse); 29709 (Rat)

Gene Symbol

GABRG2

UniProt

P22723 (Mouse), P18508 (Rat)

Additional GABA-A R gamma 2 Products

Product Documents for GABA-A R gamma 2 Antibody - Azide and BSA Free

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Product Specific Notices for GABA-A R gamma 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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