GABA-A R gamma 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-190
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Knockdown Validated, Western Blot
Cited:
IF/IHC, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fusion protein from the cytoplasmic loop of the gamma 2 subunit. Accession # P18508
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:32732906). Mouse reactivity reported in scientific literature (PMID: 23922756). Human reactivity reported in scientific literature (PMID: 24668190).
Specificity
Specific for endogenous levels of the ~46 kDa gamma 2-subunit of the GABAA receptor.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GABA-A R gamma 2 Antibody
Western Blot: GABA-A R gamma 2 Antibody [NB300-190]
Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190]
Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.Western Blot: GABA-A R gamma 2 Antibody [NB300-190] -
Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Pattern of YFP gamma2 expression in retinas of Thy1-YFP gamma2 transgenic mice.(A) Maximum intensity projections (MIP) of confocal image stacks (2 µm total thickness) of vertical slices of Thy1-YFP gamma2 retinas at postnatal day (P)12 & P21. Diffuse fluorescence expression in cell bodies within the ganglion cell layer (GCL) at both ages, & punctate expression throughout both OFF & ON layers of the inner plexiform layer (IPL) are apparent. There was no expression in the outer plexiform layer (OPL) or the outer nuclear layer (ONL) at either age. (B) Images of MIPs (∼20 µm thick) at various depths of a P21 flat mount Thy1-YFP gamma2 retina. Inserts are 3× magnification of the images. (C) Western blot of whole brain lysates from Thy1-YFP gamma2 (Tg) & wildtype (WT) mice. YFP gamma2 (arrowhead) was detected with anti-GABAAR gamma2 (upper) & anti-GFP (lower). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GABA-A R gamma 2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100
Western Blot
1:1000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 25716832). Knockdown validation (PMID: 31747610).
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GABA-A R gamma 2
Long Name
gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide
Alternate Names
GABAAR gamma 2, GABAARg2, GABRG2
Gene Symbol
GABRG2
UniProt
Additional GABA-A R gamma 2 Products
Product Documents for GABA-A R gamma 2 Antibody
Product Specific Notices for GABA-A R gamma 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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