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GABA-A R gamma 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-190

Novus Biologicals, part of Bio-Techne
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NB300-190

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Knockdown Validated, Western Blot

Cited:

IF/IHC, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Fusion protein from the cytoplasmic loop of the gamma 2 subunit. Accession # P18508

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32732906). Mouse reactivity reported in scientific literature (PMID: 23922756). Human reactivity reported in scientific literature (PMID: 24668190).

Specificity

Specific for endogenous levels of the ~46 kDa gamma 2-subunit of the GABAA receptor.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-A R gamma 2 Antibody

Western Blot: GABA-A R gamma 2 Antibody [NB300-190]

Western Blot: GABA-A R gamma 2 Antibody [NB300-190]

Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.
Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190]

Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190]

Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.
GABA-A R gamma 2 Antibody

Western Blot: GABA-A R gamma 2 Antibody [NB300-190] -

Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Pattern of YFP gamma2 expression in retinas of Thy1-YFP gamma2 transgenic mice.(A) Maximum intensity projections (MIP) of confocal image stacks (2 µm total thickness) of vertical slices of Thy1-YFP gamma2 retinas at postnatal day (P)12 & P21. Diffuse fluorescence expression in cell bodies within the ganglion cell layer (GCL) at both ages, & punctate expression throughout both OFF & ON layers of the inner plexiform layer (IPL) are apparent. There was no expression in the outer plexiform layer (OPL) or the outer nuclear layer (ONL) at either age. (B) Images of MIPs (∼20 µm thick) at various depths of a P21 flat mount Thy1-YFP gamma2 retina. Inserts are 3× magnification of the images. (C) Western blot of whole brain lysates from Thy1-YFP gamma2 (Tg) & wildtype (WT) mice. YFP gamma2 (arrowhead) was detected with anti-GABAAR gamma2 (upper) & anti-GFP (lower). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GABA-A R gamma 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 25716832). Knockdown validation (PMID: 31747610).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R gamma 2

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide

Alternate Names

GABAAR gamma 2, GABAARg2, GABRG2

Entrez Gene IDs

2566 (Human); 14406 (Mouse); 29709 (Rat)

Gene Symbol

GABRG2

UniProt

Additional GABA-A R gamma 2 Products

Product Documents for GABA-A R gamma 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GABA-A R gamma 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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