GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09047
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, X-Ray Crystallography
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 1F4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised against alpha 1 beta 2 gamma 2 GABA-A receptor.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free [NBP3-09047]](https://resources.bio-techne.com/images/products/GABA-AR-alpha-1-Antibody-1F4-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09047-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free [NBP3-09047]")
Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free [NBP3-09047]
Immunocytochemistry/Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric [NBP3-09047] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1F4 (NBP3-09047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
X-Ray Crystallography
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
FSEC: High affinity and specificity of the 1F4 antibody to alpha 1 beta 2 gamma 2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018).
FSEC: High affinity and specificity of the 1F4 antibody to alpha 1 beta 2 gamma 2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GABA-A R alpha 1
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide
Alternate Names
EJM, GABAAR alpha 1, GABAARa1, GABRA1
Gene Symbol
GABRA1
Additional GABA-A R alpha 1 Products
Product Documents for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free
Product Specific Notices for GABA-AR alpha 1 Antibody (1F4) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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