GABA-AR alpha 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-191
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Knockout Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P62813
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 22927941)
Localization
Membrane; multi pass membrane protein.
Specificity
Specific for endogenous levels of the ~51 kDa alpha1-subunit of the GABAA receptor. Immunolabeling is absent in alpha1-subunit knockout animals.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GABA-AR alpha 1 Antibody
![Western Blot: GABA-AR alpha 1 Antibody [NB300-191]](https://resources.bio-techne.com/images/products/GABA-A-R-alpha-1-Antibody-Western-Blot-NB300-191-img0003.jpg "Western Blot: GABA-AR alpha 1 Antibody [NB300-191]")
Western Blot: GABA-AR alpha 1 Antibody [NB300-191]
Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.Applications for GABA-AR alpha 1 Antibody
Application
Recommended Usage
Immunohistochemistry-Frozen
1:100
Western Blot
1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22927941). Use in immunoprecipitation reported in scientific literature (PMID: 17404840).
Reviewed Applications
Read 1 review rated 5 using NB300-191 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GABA-A R alpha 1
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide
Alternate Names
EJM, GABAAR alpha 1, GABAARa1, GABRA1
Gene Symbol
GABRA1
UniProt
Additional GABA-A R alpha 1 Products
Product Documents for GABA-AR alpha 1 Antibody
Product Specific Notices for GABA-AR alpha 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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