GABA-AR alpha 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-207
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fusion protein from the cytoplasmic loop of the alpha 1 subunit. Accession # P62813
Reactivity Notes
It is anticipated that the antibody will react with bovine, canine, human, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Localization
Membrane; multi pass membrane protein.
Specificity
Specific for endogenous levels of the ~51 kDa alpha1-subunit of the GABAA receptor. Labeling is absent in alpha1-subunit knockout animals.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GABA-AR alpha 1 Antibody
![Western Blot: GABA-AR alpha 1 Antibody [NB300-207]](https://resources.bio-techne.com/images/products/GABA-AR-alpha-1-Antibody-Western-Blot-NB300-207-img0001.jpg "Western Blot: GABA-AR alpha 1 Antibody [NB300-207]")
Western Blot: GABA-AR alpha 1 Antibody [NB300-207]
Western Blot: GABA-AR alpha 1 Antibody [NB300-207] - Mouse forebrain lysates from wild type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.Applications for GABA-AR alpha 1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100
Western Blot
1:1000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:28536106).
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GABA-A R alpha 1
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide
Alternate Names
EJM, GABAAR alpha 1, GABAARa1, GABRA1
Gene Symbol
GABRA1
UniProt
Additional GABA-A R alpha 1 Products
Product Documents for GABA-AR alpha 1 Antibody
Product Specific Notices for GABA-AR alpha 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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