GABA-AR alpha 6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-196

Novus Biologicals, part of Bio-Techne
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NB300-196
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-A R alpha 6 Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytoplasmic loop of the alpha 6 subunit. Accession # P30191

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 17977981).

Specificity

Specific for endogenous levels of the ~57 kDa alpha 6-subunit of the GABAA receptor. Labeling is absent in alpha 6-subunit knockout animals.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 6 Antibody

Western Blot: GABA-AR alpha 6 Antibody [NB300-196]

Western Blot: GABA-AR alpha 6 Antibody [NB300-196]

Western Blot: GABA-A R alpha 6 Antibody [NB300-196] - Analysis of mouse forebrain lysates from Wild Type (Control) and a6-knockout (a6-K/O) animals showing specific immunolabeling of the ~57k a6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a6-knockout animals.
Western Blot: GABA-AR alpha 6 Antibody [NB300-196]

Western Blot: GABA-AR alpha 6 Antibody [NB300-196]

Western Blot: GABA A Receptor alpha 6 Antibody [NB300-196] - Detection of GABA-A alpha 6 receptor in wildtype cerebellum (5-7 ug/lane).

Applications for GABA-AR alpha 6 Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 17977981). Use in Immunohistochemistry reported in scientific literature (PMID: 30016665).Use in Immunohistochemistry reported in scientific literature (PMID: 30016665). Western blot a band at approximately 57 kDa is seen. Immunoprecipitation showed 53% of all GABA-A receptors in dodecylsulfate extracts of rat cerebella (150 ul).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R alpha 6

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. The alpha 6 subunit is expressed in cerebellar granule cells. Though it is concentrated at synapses, it is found on extrasynaptic membranes, as well.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-6 Polypeptide

Alternate Names

GABAAR alpha 6, GABAARa6, GABRA6

Entrez Gene IDs

2559 (Human); 29708 (Rat)

Gene Symbol

GABRA6

UniProt

P30191 (Rat)

Additional GABA-A R alpha 6 Products

Product Documents for GABA-AR alpha 6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-AR alpha 6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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