GABA-B R2 [p Ser783] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61101

Novus Biologicals, part of Bio-Techne
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NB100-61101
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GABA-B R2 Antibodies »

Product Summary for GABA-B R2 [p Ser783] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH. Accession # O88871

Modification

p Ser783

Specificity

Specific for endogenous levels of the ~102 kDa GABA-B R2 phosphorylated at Ser783. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-B R2 [p Ser783] Antibody

Western Blot: GABA-B R2 [p Ser783] Antibody [NB100-61101]

Western Blot: GABA-B R2 [p Ser783] Antibody [NB100-61101]

Western Blot: GABA-B R2 [p Ser783] Antibody [NB100-61101] - Rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for GABA-B R2 [p Ser783] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-B R2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Alternate Names

GABABR2, GABBR2, GPR51, GPRC3B

Entrez Gene IDs

25451 (Rat)

Gene Symbol

GABRB2

UniProt

O88871

Additional GABA-B R2 Products

Product Documents for GABA-B R2 [p Ser783] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GABA-B R2 [p Ser783] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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