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GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25474

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25474

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline

Predicted:

Bovine (98%), Chicken (90%), Chimpanzee (100%), Porcine (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1095

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAD1/GAD67. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] -

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. GAD67 stained by GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] -

Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] -

Western Blot: GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free [NBP3-25474] - Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (NBP3-25474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Gene Symbol

GAD1

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAD1/GAD67 Antibody (HL1095) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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