GADD34 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-778

Novus Biologicals, part of Bio-Techne
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NB100-778
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GADD34 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AAALDLSGRRG corresponding to C-Terminus according to NP_055145.3.

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GADD34 Antibody

Western Blot: GADD34 Antibody [NB100-778]

Western Blot: GADD34 Antibody [NB100-778]

Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]

Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]

Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778]

Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778]

Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Applications for GADD34 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2-4ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in HepG2 lysates (calculated MW of 73.4 kDa band according to NP_055145.2). IHC-P: Human liver shows cytoplasmic staining consistent with ER in the hepatocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-778 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GADD34

GADD34 is a phosphatase whose expression is increased by exposure of cells to DNA damage or stress induced growth arrest. Induction is correlated with apoptosis following exposure to ionizing radiation.

Long Name

Protein phosphatase 1 regulatory subunit 15A

Alternate Names

PPP1R15A

Entrez Gene IDs

23645 (Human)

Gene Symbol

PPP1R15A

UniProt

NP_055145. (Human)

Additional GADD34 Products

Product Documents for GADD34 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GADD34 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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