GAGE1 Antibody (4G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00002543-M04

Novus Biologicals, part of Bio-Techne
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H00002543-M04
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GAGE1 Antibodies »

Product Specifications

Immunogen

GAGE1 (NP_001459.2, 1 a.a. ~ 139 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSWRGRSTYYWPRPRRYVQPPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAAQEGEDEGASAGQGPKPEADSQEQGHPQTGCECEDGPDGQEMDPPNPEEVKTPEEEMRSHYVAQTGILWLLMNNCFLNLSPRKP

Specificity

This product is specific for Human GAGE1 monoclonal antibody (M04), clone 4G6 [Gene ID: 2543].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GAGE1 Antibody (4G6)

Western Blot: GAGE1 Antibody (4G6) [H00002543-M04]

Western Blot: GAGE1 Antibody (4G6) [H00002543-M04]

Western Blot: GAGE1 Antibody (4G6) [H00002543-M04] - Analysis of GAGE1 expression in transfected 293T cell line by GAGE1 monoclonal antibody (M04), clone 4G6. Lane 1: GAGE1 transfected lysate (Predicted MW: 15.6 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: GAGE1 Antibody (4G6) [H00002543-M04] - Detection limit for recombinant GST tagged GAGE1 is 0.3 ng/ml as a capture antibody.

Applications for GAGE1 Antibody (4G6)

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GAGE1

GAGE1 belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs.The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene. [provided by RefSeq]

Alternate Names

Antigen MZ2-F, Cancer/testis antigen 4.1, G antigen 1, GAGE-1, member 1, MGC33825, MZ2-F antigen

Gene Symbol

GAGE1

UniProt

NP_001459.2 (Human)

Additional GAGE1 Products

Product Documents for GAGE1 Antibody (4G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAGE1 Antibody (4G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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