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GAGE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00002543-B01P

Novus Biologicals, part of Bio-Techne
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H00002543-B01P

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

GAGE1 (NP_001459.2, 1 a.a. - 139 a.a.) full-length human protein. MSWRGRSTYYWPRPRRYVQPPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAAQEGEDEGASAGQGPKPEADSQEQGHPQTGCECEDGPDGQEMDPPNPEEVKTPEEEMRSHYVAQTGILWLLMNNCFLNLSPRKP

Specificity

GAGE1 - G antigen 1,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for GAGE1 Antibody

Western Blot: GAGE1 Antibody [H00002543-B01P]

Western Blot: GAGE1 Antibody [H00002543-B01P]

Western Blot: GAGE1 Antibody [H00002543-B01P] - Analysis of GAGE1 expression in transfected 293T cell line by GAGE1 polyclonal antibody. Lane 1: GAGE1 transfected lysate(15.29 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: GAGE1 Antibody [H00002543-B01P]

Immunohistochemistry-Paraffin: GAGE1 Antibody [H00002543-B01P]

Immunohistochemistry-Paraffin: GAGE1 Antibody [H00002543-B01P] - Analysis of purified antibody to GAGE1 on formalin-fixed paraffin-embedded human testis. (antibody concentration 3 ug/ml)

Applications for GAGE1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IHC-P.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GAGE1

This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs.The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene. [provided by RefSeq]

Alternate Names

Antigen MZ2-F, Cancer/testis antigen 4.1, G antigen 1, GAGE-1, member 1, MGC33825, MZ2-F antigen

Entrez Gene IDs

2543 (Human)

Gene Symbol

GAGE1

Additional GAGE1 Products

Product Documents for GAGE1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAGE1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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