Galanin R1/GALR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57079

Novus Biologicals, part of Bio-Techne
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NB100-57079
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Galanin R1/GALR1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HLSDTKENKSRID corresponding to C-Terminus according to NP_001471.1.

Epitope

HLSDTKENKSRID

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Galanin R1/GALR1 Antibody

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079] - Analysis of GALR1 (C Term) in human heart lysate (35ug protein in RIPA buffer) using this antibody at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Galanin R1/GALR1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in human heart lysates (calculated MW of 38.9 kDa band according to NP_001471.1). An additional band of unknown identity was also consistently observed at 150 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galanin R1/GALR1

The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Long Name

Galanin Receptor Type 1

Alternate Names

GAL1, GalaninR1, GALNR1, GALR1

Entrez Gene IDs

2587 (Human)

Gene Symbol

GALR1

UniProt

NP_001471.1 (Human)

Additional Galanin R1/GALR1 Products

Product Documents for Galanin R1/GALR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Galanin R1/GALR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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