Galanin R1/GALR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57079

Novus Biologicals, part of Bio-Techne
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NB100-57079
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Galanin R1/GALR1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HLSDTKENKSRID corresponding to C-Terminus according to NP_001471.1.

Epitope

HLSDTKENKSRID

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Galanin R1/GALR1 Antibody

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079] - Analysis of GALR1 (C Term) in human heart lysate (35ug protein in RIPA buffer) using this antibody at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079]

Western Blot: Galanin R1/GALR1 Antibody [NB100-57079] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Galanin R1/GALR1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in human heart lysates (calculated MW of 38.9 kDa band according to NP_001471.1). An additional band of unknown identity was also consistently observed at 150 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Galanin R1/GALR1

The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

Long Name

Galanin Receptor Type 1

Alternate Names

GAL1, GalaninR1, GALNR1, GALR1

Entrez Gene IDs

2587 (Human)

Gene Symbol

GALR1

UniProt

NP_001471.1 (Human)

Additional Galanin R1/GALR1 Products

Product Documents for Galanin R1/GALR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Galanin R1/GALR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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