GALE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87066

Novus Biologicals, part of Bio-Techne
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NBP1-87066
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GALE Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGIPNNLMPYVSQVAIGRREALNVFGNDYDTEDGTGVRDYIHVVDLAKGHIAALRKLKEQCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GALE Antibody

Western Blot: GALE Antibody [NBP1-87066]

Western Blot: GALE Antibody [NBP1-87066]

Western Blot: GALE Antibody [NBP1-87066] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: GALE Antibody [NBP1-87066]

Immunocytochemistry/ Immunofluorescence: GALE Antibody [NBP1-87066]

Immunocytochemistry/Immunofluorescence: GALE Antibody [NBP1-87066] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066]

Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066]

Immunohistochemistry-Paraffin: GALE Antibody [NBP1-87066] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
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Applications for GALE Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-87066 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALE

GALE encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

Alternate Names

EC 5.1.3, EC 5.1.3.2, FLJ95174, FLJ97302, Galactowaldenase, short chain dehydrogenase/reductase family 1E, member 1, UDP-, UDP galactose-4'-epimerase, UDP-galactose 4-epimerase, UDP-galactose-4-epimerase, UDP-glucose 4-epimerase

Gene Symbol

GALE

Additional GALE Products

Product Documents for GALE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GALE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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