GALK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99783

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GALK1 Antibodies »

Product Summary for GALK1 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human GALK1 (Accession#: P51570; Met1-Leu392)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GALK1 Antibody

Western Blot: GALK1 Antibody [NBP2-99783]

Western Blot: GALK1 Antibody [NBP2-99783]

Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: GALK1 knockout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa
Immunocytochemistry/ Immunofluorescence: GALK1 Antibody [NBP2-99783]

Immunocytochemistry/ Immunofluorescence: GALK1 Antibody [NBP2-99783]

Immunocytochemistry/Immunofluorescence: GALK1 Antibody [NBP2-99783] - Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- HG3C-GALK1 polyclonal antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.
Western Blot: GALK1 Antibody [NBP2-99783]

Western Blot: GALK1 Antibody [NBP2-99783]

Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa (We are unsure as to the identity of these extra bands).
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Applications for GALK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALK1

The GALK1 gene encodes for a major enzyme involved in the metabolism of galactose. The protein coded is 392 amino acids long at a mass of around 42 kDA. A lack of the protein coded by the GALK1 gene results in infantile congenital cataracts and adult presenile cataracts. The GALK1 gene has also been linked to pneumonia, jaundice, hepatoblastoma, citrin deficiency, galactosemia, down syndrome, liver disease, ovarian cancer, and mevalonic aciduria. It interacts with DMWD, OTUD4, ATG2A, ATG10, AGO4 to function in galactose metabolism, the Leloir pathway, and colanic acid building blocks biosynthesis.

Long Name

Galactokinase 1

Alternate Names

GALK, HEL-S-19

Gene Symbol

GALK1

Additional GALK1 Products

Product Documents for GALK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GALK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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