gamma-2 Tubulin Antibody (4F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00027175-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all gamma-2 Tubulin Antibodies »

Product Specifications

Immunogen

TUBG2 (NP_057521, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGA

Reactivity Notes

Other species not tested.

Localization

Centrosomal

Specificity

TUBG2 - tubulin, gamma 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for gamma-2 Tubulin Antibody (4F6)

Western Blot: gamma-2 Tubulin Antibody (4F6) [H00027175-M01]

Western Blot: gamma-2 Tubulin Antibody (4F6) [H00027175-M01]

Western Blot: gamma-2 Tubulin Antibody (4F6) [H00027175-M01] - TUBG2 monoclonal antibody (M01), clone 4F6 Analysis of TUBG2 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: gamma-2 Tubulin Antibody (4F6) [H00027175-M01]

Immunocytochemistry/ Immunofluorescence: gamma-2 Tubulin Antibody (4F6) [H00027175-M01]

Immunocytochemistry/Immunofluorescence: gamma-2 Tubulin Antibody (4F6) [H00027175-M01] - Analysis of monoclonal antibody to TUBG2 on HeLa cell. Antibody concentration 25 ug/ml.
Sandwich ELISA: gamma-2 Tubulin Antibody (4F6) [H00027175-M01] - Detection limit for recombinant GST tagged TUBG2 is approximately 0.03ng/ml as a capture antibody.

Applications for gamma-2 Tubulin Antibody (4F6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: gamma-2 Tubulin

Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Long Name

Tubulin gamma-2 chain

Alternate Names

Gamma-2-tubulin, TUBG2

Entrez Gene IDs

27175 (Human)

Gene Symbol

TUBG2

OMIM

605785 (Human)

UniProt

NP_057521 (Human)

Additional gamma-2 Tubulin Products

Product Documents for gamma-2 Tubulin Antibody (4F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for gamma-2 Tubulin Antibody (4F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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