Gamma Adaptin Antibody (PSH02-65)

Western Blot: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365]

Western Blot: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365] - Western blot analysis of Gamma Adaptin on different lysates with Rabbit anti-Gamma Adaptin antibody (NBP3-32365) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: Raji cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: A549 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: RAW264.7 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse kidney tissue lysate (40 µg/Lane)
Lane 12: Mouse liver tissue lysate (40 µg/Lane)
Lane 13: Rat kidney tissue lysate (40 µg/Lane)
Lane 14: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 91 kDa
Observed band size: 91 kDa

Exposure time: 1 minute 46 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365]

Immunohistochemistry: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Gamma Adaptin antibody (NBP3-32365) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32365) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.