gamma Catenin [p Tyr550] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23198
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for gamma Catenin [p Tyr550] Antibody
Immunogen
Phospho-gamma Catenin (Tyr-550) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 550 of human gamma Catenin. This peptide sequence is highly conserved in rat and mouse gamma Catenin. [UniProt# P14923]
Modification
p Tyr550
Specificity
This antibody was cross-adsorbed to both phospho-tyrosine coupled to agarose and to dephosphorylated gamma Catenin (Tyr-550) peptide before affinity purification using phospho-gamma Catenin (Tyr-550) peptide (without carrier). The antibody detects an 84kDa protein corresponding to the molecular mass of gamma Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for gamma Catenin [p Tyr550] Antibody
![gamma Catenin [p Tyr550] Antibody](https://resources.bio-techne.com/images/products/nbp3-23198_rabbit-gamma-catenin-p-tyr550-pab-199202312403722.jpg "Immunocytochemistry/Immunofluorescence: gamma Catenin [p Tyr550] Antibody [NBP3-23198] -")
Immunocytochemistry/Immunofluorescence: gamma Catenin [p Tyr550] Antibody [NBP3-23198] -
Immunocytochemistry/Immunofluorescence: gamma Catenin [p Tyr550] Antibody [NBP3-23198] - Immunocytochemical labeling of phosphorylated gamma Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal gamma Catenin (CM1111) or rabbit polyclonal gamma Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.![gamma Catenin [p Tyr550] Antibody](https://resources.bio-techne.com/images/products/nbp3-23198_rabbit-gamma-catenin-p-tyr550-pab-19920231410128.jpg "Western Blot: gamma Catenin [p Tyr550] Antibody [NBP3-23198] -")
Western Blot: gamma Catenin [p Tyr550] Antibody [NBP3-23198] -
Western Blot: gamma Catenin [p Tyr550] Antibody [NBP3-23198] - Western blot analysis of anti-gamma Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with gamma Catenin (a.a. 545-555), gamma Catenin (Tyr-550) or gamma Catenin (C-terminal) antibodies. The anti-gamma Catenin (Tyr-550) was used in the presence of gamma Catenin (Tyr-550) (lane 5) or gamma Catenin (Tyr-644) (lane 6) peptides.Applications for gamma Catenin [p Tyr550] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: gamma Catenin
Alternate Names
catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80kDa, Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB
Gene Symbol
JUP
Additional gamma Catenin Products
Product Documents for gamma Catenin [p Tyr550] Antibody
Product Specific Notices for gamma Catenin [p Tyr550] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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