gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12540

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all gamma-Glutamylcyclotransferase/CRF21 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human gamma-Glutamylcyclotransferase/CRF21/GGCT (Accession#: O75223-1; Met1-Leu188)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]

Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]

Western Blot: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]

Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]

Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (1:10000).
Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]

Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540]

Immunohistochemistry-Paraffin: gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody [NBP3-12540] - Staining of human GGCT in human lung cancer with rabbit polyclonal antibody (1:10000).
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Applications for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma-Glutamylcyclotransferase/CRF21

CRF21 (Cytochrome c-releasing factor 21; also C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97-188. Full-length human CRF21 is 82% aa identical to mouse CRF21.

Long Name

Cytochrome c Releasing Factor 21

Alternate Names

C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017

Gene Symbol

GGCT

Additional gamma-Glutamylcyclotransferase/CRF21 Products

Product Documents for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for gamma-Glutamylcyclotransferase/CRF21/GGCT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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