gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20099

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-31A-10

Format

Azide and BSA Free

Concentration

1 mg/ml

View all gamma-Synuclein Antibodies »

Product Summary for gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

Immunogen

The KLH-conjugated peptide "YLAPSGPSGTLK", derived from Gamma-synuclein. Gamma-synulein plays a role in neurofilament network integrity.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

Flow Cytometry: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] -

Flow Cytometry: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] - Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (NBP3-20099, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] -

Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-31A-10 (NBP3-20099) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20099, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Ab can be used in immunoMRM.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: gamma-Synuclein

Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. May be a centrosome-associated protein. Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. Belongs to the synuclein family.

Alternate Names

BCSG1, PRSN, SNCG, Synoretin, Synuclein-gamma

Gene Symbol

SNCG

Additional gamma-Synuclein Products

Product Documents for gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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