GAP-43 Antibody (3H14)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05535
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # 3H14
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length Human GAP-43
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GAP-43 Antibody (3H14)
Western Blot: GAP-43 Antibody (3H14) [NBP3-05535]
Western Blot: GAP-43 Antibody [NBP3-05535] - Western blot analysis of different tissue and cell lysates using GAP-43 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP-43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (3H14) [NBP3-05535]
Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP3-05535] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with GAP-43 Antibody at a dilution of 1:1,000, in red, and costained with chicken pAb to MAP2 at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP-43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.Applications for GAP-43 Antibody (3H14)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000-1:5000
Immunohistochemistry
1:1000-5000
Western Blot
1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: GAP-43
Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.
Long Name
Growth Associated Protein 43
Alternate Names
B-50, Basp2, GAP43, Neuromodulin
Gene Symbol
GAP43
Additional GAP-43 Products
Product Documents for GAP-43 Antibody (3H14)
Product Specific Notices for GAP-43 Antibody (3H14)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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