GAPDH Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56875
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Canine, Drosophila, Feline, Hamster, Primate
Cited:
Human, Mouse, Rat, Porcine, Canine, Feline, Hamster, Insect - Drosophila, Primate
Predicted:
Gerbil (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Amino acids 73-87 PITIFQERDPSKIKW of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen of this GAPDH antibody.
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID:32764569). Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in baboon, chimp and macaque; salamander(86%). .
Marker
Cytosolic Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GAPDH Antibody
Western Blot: GAPDH Antibody [NB100-56875]
Western Blot: GAPDH Antibody [NB100-56875] - Analysis of GAPDH using this antibody at 1:500 in nuclear and cytoplasmic fractions made from Drosophila head extracts. Data courtesy of Dr. Jerry Lin, University of Wisconsin-Madison.Western Blot: GAPDH Antibody [NB100-56875]
Western Blot: GAPDH Antibody [NB100-56875] - Analysis of GAPDH in the multiple human tumor cell line lysate INSTA-Blot using this antibody. 25 ug/ml. Theoretical molecular weight: 36 kDa.Western Blot: GAPDH Antibody [NB100-56875]
Western Blot: GAPDH Antibody [NB100-56875] - Analysis using HeLa cells. Theoretical molecular weight: 36 kDa.Applications for GAPDH Antibody
Application
Recommended Usage
Simple Western
1:500
Western Blot
1:500-1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Use in immunoblotting reported in scientific literature (PMID: 28545464). Use in ICC/IF was reported in scientific literature (PMID: 31312260). Use in Immunohistochemistry reported in scientific literature (PMID:32123074).
Reviewed Applications
Read 5 reviews rated 4.8 using NB100-56875 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Gene Symbol
GAPDH
UniProt
Additional GAPDH Products
Product Documents for GAPDH Antibody
Product Specific Notices for GAPDH Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...