Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E10

Format

BSA Free

Concentration

1 mg/ml

View all GARS Antibodies »

Product Specifications

Immunogen

Recombinant human GARS (43-289aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GARS Antibody (4E10) - BSA Free

Western Blot: GARS Antibody (4E10)BSA Free [NBP2-42679]

Western Blot: GARS Antibody (4E10)BSA Free [NBP2-42679]

Western Blot: GARS Antibody (4E10) [NBP2-42679] - The cell lysates of Hela and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS antibody 1:500. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: GARS Antibody (4E10) - BSA Free [NBP2-42679]

Immunocytochemistry/ Immunofluorescence: GARS Antibody (4E10) - BSA Free [NBP2-42679]

Immunocytochemistry/Immunofluorescence: GARS Antibody (4E10) [NBP2-42679] - Analysis of GARS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GARS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for GARS Antibody (4E10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GARS

GARS encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq]

Alternate Names

AP-4-A synthetase, Charcot-Marie-Tooth neuropathy 2D, Charcot-Marie-Tooth neuropathy, neuronal type, D, Diadenosine tetraphosphate synthetase, DSMAV, glycine tRNA ligase, Glycine--tRNA ligase, glycyl-tRNA synthetase, GlyRSEC 6.1.1.14, HMN5, SMAD1CMT2D

Gene Symbol

GARS1

UniProt

NP_002038 (Human)

Additional GARS Products

Product Documents for GARS Antibody (4E10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GARS Antibody (4E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.