GARS Antibody (5Q5D7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15293
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5Q5D7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 640-739 of human GARS (P41250). RHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GARS Antibody (5Q5D7)
Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] -
Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] - Western blot analysis of Jurkat, using GARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] -
Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] - Western blot analysis of extracts from wild type(WT) and GARS knockout (KO) 293T cells, using GARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] -
Western Blot: GARS Antibody (5Q5D7) [NBP3-15293] - Western blot analysis of various lysates, using [KO Validated] GARS Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.
Applications for GARS Antibody (5Q5D7)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GARS
Alternate Names
AP-4-A synthetase, Charcot-Marie-Tooth neuropathy 2D, Charcot-Marie-Tooth neuropathy, neuronal type, D, Diadenosine tetraphosphate synthetase, DSMAV, glycine tRNA ligase, Glycine--tRNA ligase, glycyl-tRNA synthetase, GlyRSEC 6.1.1.14, HMN5, SMAD1CMT2D
Gene Symbol
GARS1
Additional GARS Products
Product Documents for GARS Antibody (5Q5D7)
Product Specific Notices for GARS Antibody (5Q5D7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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