GATA-3 Antibody (CL8660)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88917

Novus Biologicals, part of Bio-Techne
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NBP2-88917
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL8660

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GATA-3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: DQPRWVSHHHPAVLNGQHPDTHHP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GATA-3 Antibody (CL8660)

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.
Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]

Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]

Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.
Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for GATA-3 Antibody (CL8660)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (CL8660)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-3 Antibody (CL8660)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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