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GATA-5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55399

Novus Biologicals, part of Bio-Techne
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NB100-55399

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-ELAARPGWAQTATAD corresponding to internal region according to NP_536721.1.

Epitope

ELAARPGWAQTATAD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-5 Antibody

Western Blot: GATA-5 Antibody [NB100-55399]

Western Blot: GATA-5 Antibody [NB100-55399]

Western Blot: GATA-5 Antibody [NB100-55399] - (0.3ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GATA-5 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates of liver cell line HepG2 (calculated MW of 41.3 kDa band according to NP_536721.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-5

The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity.

Alternate Names

GATA5

Entrez Gene IDs

140628 (Human)

Gene Symbol

GATA5

Additional GATA-5 Products

Product Documents for GATA-5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GATA-5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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