GBE1 Antibody (OTI1D11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02245
![Flow Cytometry: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Flow-Cytometry-NBP2-02245-img0001.jpg "Flow Cytometry: GBE1 Antibody (OTI1D11) [NBP2-02245]")
![Western Blot: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Western-Blot-NBP2-02245-img0009.jpg "Western Blot: GBE1 Antibody (OTI1D11) [NBP2-02245]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1D11
Concentration
1.1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
80.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GBE1 Antibody (OTI1D11)
Western Blot: GBE1 Antibody (OTI1D11) [NBP2-02245]
Western Blot: GBE1 Antibody (OTI1D11) [NBP2-02245] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).![Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Immunocytochemistry-Immunofluorescence-NBP2-02245-img0007.jpg "Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) [NBP2-02245]")
Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) [NBP2-02245]
Immunocytochemistry/Immunofluorescence: GBE1 Antibody (OTI1D11) [NBP2-02245] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.![Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Immunohistochemistry-Paraffin-NBP2-02245-img0006.jpg "Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]")
Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]
Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245] - Staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody.![Western Blot: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Western-Blot-NBP2-02245-img0002.jpg "Western Blot: GBE1 Antibody (OTI1D11) [NBP2-02245]")
![Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Immunocytochemistry-Immunofluorescence-NBP2-02245-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) [NBP2-02245]")
![Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Immunohistochemistry-Paraffin-NBP2-02245-img0004.jpg "Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]")
![Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Immunohistochemistry-Paraffin-NBP2-02245-img0005.jpg "Immunohistochemistry-Paraffin: GBE1 Antibody (OTI1D11) [NBP2-02245]")
![Immunoprecipitation: GBE1 Antibody (OTI1D11) [NBP2-02245]](https://resources.bio-techne.com/images/products/GBE1-Antibody-OTI1D11-Immunoprecipitation-NBP2-02245-img0010.jpg "Immunoprecipitation: GBE1 Antibody (OTI1D11) [NBP2-02245]")
Applications for GBE1 Antibody (OTI1D11)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:50-100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Immunoprecipitation
2ug/500ul
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GBE1
Alternate Names
1,4-alpha-glucan-branching enzyme, amylo-(1,4 to 1,6) transglucosidase, amylo-(1,4 to 1,6) transglycosylase, Brancher enzyme, EC 2.4.1.18, GBE, glucan (1,4-alpha), branching enzyme 1, glycogen branching enzyme, Glycogen-branching enzyme
Entrez Gene IDs
2632 (Human)
Gene Symbol
GBE1
Additional GBE1 Products
Product Documents for GBE1 Antibody (OTI1D11)
Product Specific Notices for GBE1 Antibody (OTI1D11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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