GBE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85877

Novus Biologicals, part of Bio-Techne
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NBP1-85877
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GBE1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LCPDSITIAEDVSGMPALCSPISQGGGGFDYRLAMAIPDKWIQLLKEFKDEDWNMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSLAFWLMDAEMY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GBE1 Antibody

Western Blot: GBE1 Antibody [NBP1-85877]

Western Blot: GBE1 Antibody [NBP1-85877]

Western Blot: GBE1 Antibody [NBP1-85877] - Analysis using Anti-GBE1 antibody NBP1-85877 (A) shows similar pattern to independent antibody NBP1-85876 (B).
Immunocytochemistry/ Immunofluorescence: GBE1 Antibody [NBP1-85877]

Immunocytochemistry/ Immunofluorescence: GBE1 Antibody [NBP1-85877]

Immunocytochemistry/Immunofluorescence: GBE1 Antibody [NBP1-85877] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GBE1 Antibody [NBP1-85877]

Immunohistochemistry-Paraffin: GBE1 Antibody [NBP1-85877]

Immunohistochemistry-Paraffin: GBE1 Antibody [NBP1-85877] - Staining in human adrenal gland and pancreas tissues using anti-GBE1 antibody. Corresponding GBE1 RNA-seq data are presented for the same tissues.
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Applications for GBE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GBE1

GBE1 is encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).

Alternate Names

1,4-alpha-glucan-branching enzyme, amylo-(1,4 to 1,6) transglucosidase, amylo-(1,4 to 1,6) transglycosylase, Brancher enzyme, EC 2.4.1.18, GBE, glucan (1,4-alpha), branching enzyme 1, glycogen branching enzyme, Glycogen-branching enzyme

Gene Symbol

GBE1

Additional GBE1 Products

Product Documents for GBE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GBE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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