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GBF1 Antibody (4B2)

Novus Biologicals, part of Bio-Techne | Catalog # H00008729-M04

Novus Biologicals, part of Bio-Techne
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H00008729-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

GBF1 (NP_004184.1, 101 a.a. ~ 209 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. THEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGT

Specificity

This product is specific for Human GBF1 monoclonal antibody (M04), clone 4B2 [Gene ID: 8729].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GBF1 Antibody (4B2)

Western Blot: GBF1 Antibody (4B2) [H00008729-M04]

Western Blot: GBF1 Antibody (4B2) [H00008729-M04]

Western Blot: GBF1 Antibody (4B2) [H00008729-M04] - Analysis of GBF1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: GBF1 Antibody (4B2) [H00008729-M04]

Immunocytochemistry/ Immunofluorescence: GBF1 Antibody (4B2) [H00008729-M04]

Immunocytochemistry/Immunofluorescence: GBF1 Antibody (4B2) [H00008729-M04] - Analysis of monoclonal antibody to GBF1 on HeLa cell. Antibody concentration 10 ug/ml

Applications for GBF1 Antibody (4B2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GBF1

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Long Name

Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

Alternate Names

BFA-resistant GEF 1, KIAA0248

Gene Symbol

GBF1

Additional GBF1 Products

Product Documents for GBF1 Antibody (4B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GBF1 Antibody (4B2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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