GBF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06526

Novus Biologicals, part of Bio-Techne
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NBP1-06526
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all GBF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1809 and 1859 of human golgi-specific brefeldin A resistance factor 1 using the numbering given in entry NP_004184.1 (GeneID 8729).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

206 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GBF1 Antibody

Immunohistochemistry: GBF1 Antibody [NBP1-06526]

Immunohistochemistry: GBF1 Antibody [NBP1-06526]

Immunohistochemistry: GBF1 Antibody [NBP1-06526] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GBF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunoprecipitation: GBF1 Antibody [NBP1-06526]

Immunoprecipitation: GBF1 Antibody [NBP1-06526]

Immunoprecipitation: GBF1 Antibody [NBP1-06526] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NBP1-06526 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. GBF1 was also immunoprecipitated by NBP1-06525 which recognizes an upstream epitope.

Applications for GBF1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GBF1

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Long Name

Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

Alternate Names

BFA-resistant GEF 1, KIAA0248

Entrez Gene IDs

8729 (Human); 107338 (Mouse)

Gene Symbol

GBF1

UniProt

Q92538 (Human)

Additional GBF1 Products

Product Documents for GBF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GBF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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