GBF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88844

Novus Biologicals, part of Bio-Techne
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NBP1-88844
NBP1-88844-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GBF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: INQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GBF1 Antibody

Immunocytochemistry/ Immunofluorescence: GBF1 Antibody [NBP1-88844]

Immunocytochemistry/ Immunofluorescence: GBF1 Antibody [NBP1-88844]

Immunocytochemistry/Immunofluorescence: GBF1 Antibody [NBP1-88844] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GBF1 Antibody [NBP1-88844]

Immunohistochemistry-Paraffin: GBF1 Antibody [NBP1-88844]

Immunohistochemistry-Paraffin: GBF1 Antibody [NBP1-88844] - Staining of human heart muscle shows cytoplasmic positivity in myocytes.

Applications for GBF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GBF1

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Long Name

Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

Alternate Names

BFA-resistant GEF 1, KIAA0248

Entrez Gene IDs

8729 (Human)

Gene Symbol

GBF1

UniProt

Q92538 (Human)

Additional GBF1 Products

Product Documents for GBF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GBF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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