GBX2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55393
![Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]](https://resources.bio-techne.com/images/products/GBX2-Antibody-Immunohistochemistry-Paraffin-NB100-55393-img0007.jpg "Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]")
![Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]](https://resources.bio-techne.com/images/products/GBX2-Antibody-Immunohistochemistry-Paraffin-NB100-55393-img0008.jpg "Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QGKDESKVEDDPK corresponding to internal region according to NP_001476.2.
Epitope
QGKDESKVEDDPK
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23144817).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GBX2 Antibody
![Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]](https://resources.bio-techne.com/images/products/GBX2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55393-img0010.jpg "Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]")
Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]
Immunocytochemistry/Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).![Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]](https://resources.bio-techne.com/images/products/GBX2-Antibody-Immunohistochemistry-Paraffin-NB100-55393-img0011.jpg "Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]")
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393]
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.![Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]](https://resources.bio-techne.com/images/products/GBX2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55393-img0009.jpg "Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]")
Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NB100-55393]
Immunocytochemistry/Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for GBX2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Peptide ELISA
Detection limit 1:18000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
IHC-P: Human placenta shows nuclear staining of selected trophoblasts.
IHC: In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. WB reactivity reported in (PMID: 23144817).
IHC: In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. WB reactivity reported in (PMID: 23144817).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GBX2
Long Name
Gastrulation Brain Homeobox 2
Alternate Names
Gastrulation and brain-specific homeobox protein 2, gastrulation brain homeo box 2, gastrulation brain homeobox 2, homeobox protein GBX-2
Gene Symbol
GBX2
UniProt
Additional GBX2 Products
Product Documents for GBX2 Antibody
Product Specific Notices for GBX2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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