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GCAP1 Antibody (2F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00002978-M04

Novus Biologicals, part of Bio-Techne
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H00002978-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

GUCA1A (NP_000400, 1 a.a. ~ 93 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNVMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPSASQYVEQMFETFDFNKDGYIDFMEYVAALSLVLKGKVEQKLR

Specificity

GUCA1A (2F7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GCAP1 Antibody (2F7)

Western Blot: GCAP1 Antibody (2F7) [H00002978-M04]

Western Blot: GCAP1 Antibody (2F7) [H00002978-M04]

Western Blot: GCAP1 Antibody (2F7) [H00002978-M04] - Analysis of GUCA1A expression in transfected 293T cell line by GUCA1A monoclonal antibody (M04), clone 2F7. Lane 1: GUCA1A transfected lysatE (22.9 KDa). Lane 2: Non-transfected lysate.
Western Blot: GCAP1 Antibody (2F7) [H00002978-M04]

Western Blot: GCAP1 Antibody (2F7) [H00002978-M04]

Western Blot: GCAP1 Antibody (2F7) [H00002978-M04] - Western blot analysis of GUCA1A over-expressed 293 cell line, cotransfected with GUCA1A Validated Chimera RNAi or non-transfected control. Blot probed with H00002978-M04. GAPDH (36.1 kDa) used as loading control.

Applications for GCAP1 Antibody (2F7)

Application
Recommended Usage

Western Blot

1:500RNAi Validation
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCAP1

The GCAP1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, thephototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasingCa(2+) concentrations follo

Alternate Names

C6orf131, chromosome 6 open reading frame 131, CORD14, GCAP 1, GCAP1photoreceptor 1, GCAPGUCA, Guanylate cyclase activator 1A, guanylate cyclase activator 1A (retina), guanylin 1, retina, guanylyl cyclase-activating protein 1, GUCA1

Entrez Gene IDs

2978 (Human)

Gene Symbol

GUCA1A

OMIM

600364 (Human)

Additional GCAP1 Products

Product Documents for GCAP1 Antibody (2F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GCAP1 Antibody (2F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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