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GCC185 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20922

Novus Biologicals, part of Bio-Techne
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NBP1-20922

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QKLTNKNNKIED corresponding to internal region according to NP_055450.1, NP_852118.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GCC185 Antibody

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - (3.8 ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]

Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GCC185 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human lymph node and testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185 kDa band according to NP_055450.1 and of 196 kDa band according to NP_852118.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Paraffin embedded Human Spleen, Small Intestine and Kidney.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCC185

The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC protein, 185-kD, GCC185GRIP and coiled-coil domain-containing 2, Golgi coiled-coil protein GCC185, GRIP and coiled-coil domain containing 2, GRIP and coiled-coil domain-containing protein 2, KIAA0336REN53, Ran-binding protein 2-like 4, RANBP2L4, Renal carcinoma antigen NY-REN-53

Entrez Gene IDs

9648 (Human)

Gene Symbol

GCC2

Additional GCC185 Products

Product Documents for GCC185 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GCC185 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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