GCC185 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20922
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QKLTNKNNKIED corresponding to internal region according to NP_055450.1, NP_852118.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GCC185 Antibody
![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]](https://resources.bio-techne.com/images/products/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-20922-img0003.jpg "Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]")
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]](https://resources.bio-techne.com/images/products/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-20922-img0001.jpg "Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]")
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - (3.8 ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]](https://resources.bio-techne.com/images/products/GCC185-Antibody-Immunohistochemistry-Paraffin-NBP1-20922-img0002.jpg "Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]")
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922]
Immunohistochemistry-Paraffin: GCC185 Antibody [NBP1-20922] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for GCC185 Antibody
Application
Recommended Usage
Immunohistochemistry
3.75 ug/ml
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human lymph node and testis lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185 kDa band according to NP_055450.1 and of 196 kDa band according to NP_852118.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Paraffin embedded Human Spleen, Small Intestine and Kidney.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GCC185
Alternate Names
185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC protein, 185-kD, GCC185GRIP and coiled-coil domain-containing 2, Golgi coiled-coil protein GCC185, GRIP and coiled-coil domain containing 2, GRIP and coiled-coil domain-containing protein 2, KIAA0336REN53, Ran-binding protein 2-like 4, RANBP2L4, Renal carcinoma antigen NY-REN-53
Entrez Gene IDs
9648 (Human)
Gene Symbol
GCC2
Additional GCC185 Products
Product Documents for GCC185 Antibody
Product Specific Notices for GCC185 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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