GCC185 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04024
![Western Blot: GCC185 Antibody [NBP2-04024]](https://resources.bio-techne.com/images/products/GCC185-Antibody-Western-Blot-NBP2-04024-img0003.jpg "Western Blot: GCC185 Antibody [NBP2-04024]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human 185 kDa Golgi coiled-coil protein using the numbering given in entry NP_852118.1 (GeneID 9648).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GCC185 Antibody
Western Blot: GCC185 Antibody [NBP2-04024]
Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).![Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024]](https://resources.bio-techne.com/images/products/GCC185-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-04024-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024]")
Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024]
Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP2-04024] - PC-3 cells (human) stained with GCC185 antibody (red). Nucleus stained with DAPI (blue). EVOS(TM) M5000 Imaging System - 63x oil objective. ICC/IF image submitted by a verified customer review.Applications for GCC185 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
This GCC185 antibody is validated for ICC/IF from a verified customer review. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Reviewed Applications
Read 1 review rated 5 using NBP2-04024 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GCC185
Alternate Names
185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC protein, 185-kD, GCC185GRIP and coiled-coil domain-containing 2, Golgi coiled-coil protein GCC185, GRIP and coiled-coil domain containing 2, GRIP and coiled-coil domain-containing protein 2, KIAA0336REN53, Ran-binding protein 2-like 4, RANBP2L4, Renal carcinoma antigen NY-REN-53
Gene Symbol
GCC2
UniProt
Additional GCC185 Products
Product Documents for GCC185 Antibody
Product Specific Notices for GCC185 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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