GCDH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16622

Novus Biologicals, part of Bio-Techne
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NBP2-16622
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCDH Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCDH. The exact sequence is proprietary.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCDH Antibody

Western Blot: GCDH Antibody [NBP2-16622]

Western Blot: GCDH Antibody [NBP2-16622]

Western Blot: GCDH Antibody [NBP2-16622] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GCDH Antibody [NBP2-16622]

Immunocytochemistry/ Immunofluorescence: GCDH Antibody [NBP2-16622]

Immunocytochemistry/Immunofluorescence: GCDH Antibody [NBP2-16622] - HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: GCDH protein stained by GCDH antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-16622]

Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-16622]

Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-16622] - Mouse liver. GCDH antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for GCDH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCDH

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Alternate Names

ACAD5, EC 1.3.99, EC 1.3.99.7, GCD, glutaryl-CoA dehydrogenase, glutaryl-CoA dehydrogenase, mitochondrial, glutaryl-Coenzyme A dehydrogenase

Gene Symbol

GCDH

UniProt

Q92947 (Human)

Additional GCDH Products

Product Documents for GCDH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GCDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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