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GCNT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93400

Novus Biologicals, part of Bio-Techne
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NB100-93400

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RDTLHLTNKKKD corresponding to internal region according to NP_004742.1.

Epitope

C-RDTLHLTNKKKD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GCNT3 Antibody

Western Blot: GCNT3 Antibody [NB100-93400]

Western Blot: GCNT3 Antibody [NB100-93400]

Western Blot: GCNT3 Antibody [NB100-93400] - HEK293 overexpressing GCNT3 and probed with (mock transfection in first lane).
Western Blot: GCNT3 Antibody [NB100-93400]

Western Blot: GCNT3 Antibody [NB100-93400]

Western Blot: GCNT3 Antibody [NB100-93400] - (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GCNT3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human lymph node lysates (calculated MW of 50.9 kDa band according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCNT3

GCNT3 is a glycosyltransferase, belonging to the glycosyltransferase 14 family. It can synthesize all known mucin beta 6 N-acetylglucosaminides and mediates core 2 and core 4 O-glycan branching, two important steps in mucin-type biosynthesis.

Alternate Names

beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3, beta1,6GlcNAc-transferase, beta1,6-N-acetylglucosaminyltransferase-M, C2/4GnTGNTM, C24GNT, C2GNT2, C2GNTM, C2GnT-M, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase, EC 2.4.1.102, EC 2.4.1.150, glucosaminyl (N-acetyl) transferase 3, mucin type, hC2GnT-M, mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Entrez Gene IDs

9245 (Human)

Gene Symbol

GCNT3

Additional GCNT3 Products

Product Documents for GCNT3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GCNT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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