GCNT3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93401
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NKFDPKVDDNALQ corresponding to internal region (near C-Terminus) according to NP_004742.1.
Epitope
C-NKFDPKVDDNALQ
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GCNT3 Antibody
![Western Blot: GCNT3 Antibody [NB100-93401]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Western-Blot-NB100-93401-img0002.jpg "Western Blot: GCNT3 Antibody [NB100-93401]")
Western Blot: GCNT3 Antibody [NB100-93401]
Western Blot: GCNT3 Antibody [NB100-93401] - HEK293 overexpressing GCNT3 and probed with (mock transfection in first lane).![Western Blot: GCNT3 Antibody [NB100-93401]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Western-Blot-NB100-93401-img0001.jpg "Western Blot: GCNT3 Antibody [NB100-93401]")
Western Blot: GCNT3 Antibody [NB100-93401]
Western Blot: GCNT3 Antibody [NB100-93401] - Analysis of GCNT3 (aa 410 to 422) in Human Duodenum lysate (35ug protein in RIPA buffer) using this antibody at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for GCNT3 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:64000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 55-60 kDa band observed in human duodenum lysates (calculated MW of 50.9 kDa band according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GCNT3
Alternate Names
beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3, beta1,6GlcNAc-transferase, beta1,6-N-acetylglucosaminyltransferase-M, C2/4GnTGNTM, C24GNT, C2GNT2, C2GNTM, C2GnT-M, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase, EC 2.4.1.102, EC 2.4.1.150, glucosaminyl (N-acetyl) transferase 3, mucin type, hC2GnT-M, mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Gene Symbol
GCNT3
UniProt
Additional GCNT3 Products
Product Documents for GCNT3 Antibody
Product Specific Notices for GCNT3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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