GCNT3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88313
![Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunohistochemistry-Paraffin-NBP1-88313-img0015.jpg "Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]")
![Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunohistochemistry-Paraffin-NBP1-88313-img0017.jpg "Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFER
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24889196).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GCNT3 Antibody
![Western Blot: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Western-Blot-NBP1-88313-img0011.jpg "Western Blot: GCNT3 Antibody [NBP1-88313]")
Western Blot: GCNT3 Antibody [NBP1-88313]
Western Blot: GCNT3 Antibody [NBP1-88313] - Analysis in human cell line A-549.![Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88313-img0014.jpg "Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]")
Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]
Immunocytochemistry/Immunofluorescence: GCNT3 Antibody [NBP1-88313] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.![Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Immunohistochemistry-Paraffin-NBP1-88313-img0016.jpg "Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]")
![Simple Western: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Simple-Western-NBP1-88313-img0007.jpg "Simple Western: GCNT3 Antibody [NBP1-88313]")
![Simple Western: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Simple-Western-NBP1-88313-img0008.jpg "Simple Western: GCNT3 Antibody [NBP1-88313]")
![Simple Western: GCNT3 Antibody [NBP1-88313]](https://resources.bio-techne.com/images/products/GCNT3-Antibody-Simple-Western-NBP1-88313-img0009.jpg "Simple Western: GCNT3 Antibody [NBP1-88313]")
Applications for GCNT3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24889196). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GCNT3
Alternate Names
beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3, beta1,6GlcNAc-transferase, beta1,6-N-acetylglucosaminyltransferase-M, C2/4GnTGNTM, C24GNT, C2GNT2, C2GNTM, C2GnT-M, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase, EC 2.4.1.102, EC 2.4.1.150, glucosaminyl (N-acetyl) transferase 3, mucin type, hC2GnT-M, mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Gene Symbol
GCNT3
Additional GCNT3 Products
Product Documents for GCNT3 Antibody
Product Specific Notices for GCNT3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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