GDF-8/Myostatin Antibody (3E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00002660-M07
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GDF8 (NP_005250, 241 a.a. ~ 310 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCS
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 26730720).
Specificity
GDF8 (3E7)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GDF-8/Myostatin Antibody (3E7)
Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07]
Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Analysis of GDF8 expression in transfected 293T cell line by GDF8 monoclonal antibody (M07), clone 3E7. Lane 1: GDF8 transfected lysatE (42.8 KDa). Lane 2: Non-transfected lysate.Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07]
Western Blot: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Analysis of GDF8 expression in LNCaP (Cat # L004V1).Immunocytochemistry/ Immunofluorescence: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] -
Immunocytochemistry/ Immunofluorescence: GDF-8/Myostatin Antibody (3E7) [H00002660-M07] - Myostatin expression in human bladder smooth muscle tissue & cells. (A) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder muscle tissue. Cell nuclei stained with DAPI (blue). 10× magnification (B) Immunofluorescence staining image of myostatin protein (red) in pediatric bladder-derived hSMCs. 20× magnification (C) Flow cytometry comparison of myostatin-positive cells in pediatric bladder-derived control & ESLUTD hSMCs. (D) Representative immunoblot by WES of active myostatin (48 kDa) & its propeptides (100, 52 kDa) in bladder-derived smooth muscle tissue & cells. GAPDH (40 kDa) as a housekeeping protein. * p ≤ 0.05. Scale bar, 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36901894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GDF-8/Myostatin Antibody (3E7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GDF-8/Myostatin
Long Name
Growth Differentiation Factor 8
Alternate Names
GDF8, MSLHP, MSTN, Myostatin
Entrez Gene IDs
2660 (Human)
Gene Symbol
MSTN
OMIM
601788 (Human)
UniProt
Additional GDF-8/Myostatin Products
Product Documents for GDF-8/Myostatin Antibody (3E7)
Product Specific Notices for GDF-8/Myostatin Antibody (3E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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