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Gemin 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90354

Novus Biologicals, part of Bio-Techne
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NBP1-90354
NBP1-90354-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPET

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gemin 5 Antibody

Western Blot: Gemin 5 Antibody [NBP1-90354]

Western Blot: Gemin 5 Antibody [NBP1-90354]

Western Blot: Gemin 5 Antibody [NBP1-90354] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-237
Immunocytochemistry/ Immunofluorescence: Gemin 5 Antibody [NBP1-90354]

Immunocytochemistry/ Immunofluorescence: Gemin 5 Antibody [NBP1-90354]

Immunocytochemistry/Immunofluorescence: Gemin 5 Antibody [NBP1-90354] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP1-90354]

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP1-90354]

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP1-90354] - Staining of human cerebellum shows strong cytoplasmic and nucleolar positivity in purkinje cells.

Applications for Gemin 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gemin 5

GEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM]

Alternate Names

DKFZp586M1824, EC 2.7.7.8, EC 5.4.2.1, gem (nuclear organelle) associated protein 5, gem-associated protein 5, gemin 5, Gemin5, MGC142174

Gene Symbol

GEMIN5

Additional Gemin 5 Products

Product Documents for Gemin 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Gemin 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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