GEMIN2 Antibody (9C8K3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15717

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15717-100ul
NBP3-15717-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9C8K3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GEMIN2 Antibodies »

Product Summary for GEMIN2 Antibody (9C8K3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GEMIN2 (O14893). MRRAELAGLKTMAWVPAESAVEELMPRLLPVEPCDLTEGFDPSVPPRTPQEYLRRVQIEAAQCPDVVVAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GEMIN2 Antibody (9C8K3)

Western Blot: GEMIN2 Antibody (9C8K3) [NBP3-15717]

Western Blot: GEMIN2 Antibody (9C8K3) [NBP3-15717]

Western Blot: GEMIN2 Antibody (9C8K3) [NBP3-15717] - Western blot analysis of extracts of various cell lines, using GEMIN2 antibody (NBP3-15717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: GEMIN2 Antibody (9C8K3) [NBP3-15717]

Immunohistochemistry-Paraffin: GEMIN2 Antibody (9C8K3) [NBP3-15717]

Immunohistochemistry-Paraffin: GEMIN2 Antibody (9C8K3) [NBP3-15717] - Immunohistochemistry of paraffin-embedded rat lung using GEMIN2 Rabbit mAb (NBP3-15717) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: GEMIN2 Antibody (9C8K3) [NBP3-15717]

Western Blot: GEMIN2 Antibody (9C8K3) [NBP3-15717]

Western Blot: GEMIN2 Antibody (9C8K3) [NBP3-15717] - Western blot analysis of extracts of various cell lines, using GEMIN2 antibody (NBP3-15717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Applications for GEMIN2 Antibody (9C8K3)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GEMIN2

SIP1 encodes one of the proteins found in the SMN complex, which consists of the suvival of motor neuron protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

Alternate Names

Component of gems 2, gemin-2, GEMIN2survival of motor neuron protein-interacting protein 1, SIP1-delta, SMN interacting protein 1-delta, SMN-interacting protein 1, survival of motor neuron protein interacting protein 1

Gene Symbol

GEMIN2

Additional GEMIN2 Products

Product Documents for GEMIN2 Antibody (9C8K3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GEMIN2 Antibody (9C8K3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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