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Geminin Antibody (S08-1F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19484

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-19484-100ul
NBP3-19484-50ul

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-1F8

Concentration

0.3 mg/ml

Product Specifications

Immunogen

Recombinant protein of human Geminin (Uniprot # O75496)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Geminin Antibody (S08-1F8)

Western Blot: Geminin Antibody (S08-1F8) [NBP3-19484]

Western Blot: Geminin Antibody (S08-1F8) [NBP3-19484]

Western Blot: Geminin Antibody (S08-1F8) [NBP3-19484] - Western blot detection of Geminin in Jurkat cell lysates using NBP3-19484 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.

Applications for Geminin Antibody (S08-1F8)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Geminin

Geminin is a multifunctional nuclear protein invloved in embyro development/differentiation, cell cycle regulation and DNA replciation. Originally defined as an inhibitor of DNA replication and substrate of the anaphase promoting complex, Geminin has also been shown to expand the neural plate in the developing embryos.

Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.

Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Gene Symbol

GMNN

Additional Geminin Products

Product Documents for Geminin Antibody (S08-1F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Geminin Antibody (S08-1F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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