Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Geminin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 160 and the C-terminus (residue 209) of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Geminin Antibody

Western Blot: Geminin Antibody [NB100-40827]

Western Blot: Geminin Antibody [NB100-40827]

Western Blot: Geminin Antibody [NB100-40827] - Detection of Human Geminin on HeLa whole cell lysate using NB100-40827. Geminin was also immunoprecipitated by another rabbit anti-Geminin antibodies and NB100-40826.
Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40827]

Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40827]

Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40827] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB

Applications for Geminin Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Geminin

Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex. It is degraded during the mitotic phase of the cell cycle, has a destruction box sequence (RRTLKVIQP), and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Further, geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Entrez Gene IDs

51053 (Human)

Gene Symbol

GMNN

UniProt

O75496 (Human)

Additional Geminin Products

Product Documents for Geminin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Geminin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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