Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71556

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Gephyrin/GPHN Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

83.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

Western Blot: Gephyrin/GPHN Antibody (OTI3B6)Azide and BSA Free [NBP2-71556]

Western Blot: Gephyrin/GPHN Antibody (OTI3B6)Azide and BSA Free [NBP2-71556]

Western Blot: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Gephyrin.
Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556]

Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556]

Immunocytochemistry/Immunofluorescence: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.
Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556]

Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556]

Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.
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Applications for Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gephyrin/GPHN

The Gephyrin (GPHN) gene encodes a neuronal assembly protein that exists in isoform 1 (736 amino acids long, 79 kDA) or isoform 2 (769 amino acids in length, approximately 83 kDA). This protein anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton through high affinity binding to a receptor subunit domain and tublin dimers. The protein coded by GPHN is also necessary for molybdenum biosynthesis. The GPHN gene has been investigated in relation to many diseases such as leukemia, cervicitis, tuberculosis, alzheimer's disease, anorexia nervosa, huntington's disease, retinitis, and herpes zoster. The GPHN gene interacts with genes DYNLL1, PFN1, PRPF4, ENAH, and SLX4 to participate in pathways that regulate metabolism of vitamins and cofactors as well as molybdenum cofactor biosynthesis.

Alternate Names

GEPH, GPH, GPHN, GPHRYN

Gene Symbol

GPHN

Additional Gephyrin/GPHN Products

Product Documents for Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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