GFER/ALR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90187

Novus Biologicals, part of Bio-Techne
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NBP1-90187
NBP1-90187-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GFER/ALR Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QQDMAQFIHLFSKFYPCEECAEDLRKRLCRNHPDTRTRACFTQWLCHLHNEVNRKLGKPDFDCSKVDERWRDGWKDGSCD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%). Human reactivity reported in scientific literature (PMID: 25012650).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GFER/ALR Antibody

Western Blot: GFER/ALR Antibody [NBP1-90187]

Western Blot: GFER/ALR Antibody [NBP1-90187]

Western Blot: GFER/ALR Antibody [NBP1-90187] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: GFER/ALR Antibody [NBP1-90187]

Immunocytochemistry/ Immunofluorescence: GFER/ALR Antibody [NBP1-90187]

Immunocytochemistry/Immunofluorescence: GFER/ALR Antibody [NBP1-90187] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.
Immunohistochemistry-Paraffin: GFER/ALR Antibody [NBP1-90187]

Immunohistochemistry-Paraffin: GFER/ALR Antibody [NBP1-90187]

Immunohistochemistry-Paraffin: GFER/ALR Antibody [NBP1-90187] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Applications for GFER/ALR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFER/ALR

The hepatotrophic factor designated augmenter of liver regeneration (ALR) is thought to be one of the factorsresponsible for the extraordinary regenerative capacity of mammalian liver. It has also been called hepaticregenerative stimulation substance (HSS). The gene resides on chromosome 16 in the interval containing the locus forpolycystic kidney disease (PKD1). The putative gene product is 42% similar to the scERV1 protein of yeast. The yeastscERV1 gene had been found to be essential for oxidative phosphorylation, the maintenance of mitochondrial genomes,and the cell division cycle. The human gene is both the structural and functional homolog of the yeast scERV1 gene.(provided by RefSeq)

Long Name

Growth Factor, Augmenter of Liver Regeneration

Alternate Names

ALR, ERV1, Hepatopoietin, HERV1, HPO

Entrez Gene IDs

2671 (Human)

Gene Symbol

GFER

UniProt

P55789

Additional GFER/ALR Products

Product Documents for GFER/ALR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GFER/ALR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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