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GGA1 Antibody (1G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00026088-M02

Novus Biologicals, part of Bio-Techne
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H00026088-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

GGA1 (AAH29388, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVRRGEATIRPPPCDDTKGGQD

Reactivity Notes

Human. Other species not tested.

Specificity

GGA1 - golgi associated, gamma adaptin ear containing, ARF binding protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GGA1 Antibody (1G8)

Western Blot: GGA1 Antibody (1G8) [H00026088-M02]

Western Blot: GGA1 Antibody (1G8) [H00026088-M02]

Western Blot: GGA1 Antibody (1G8) [H00026088-M02] - Analysis of GGA1 expression in transfected 293T cell line by GGA1 monoclonal antibody (M02), clone 1G8.Lane 1: GGA1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: GGA1 Antibody (1G8) [H00026088-M02]

Western Blot: GGA1 Antibody (1G8) [H00026088-M02]

Western Blot: GGA1 Antibody (1G8) [H00026088-M02] - Analysis of GGA1 over-expressed 293 cell line, cotransfected with GGA1 Validated Chimera RNAi ( Cat # H00026088-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GGA1 monoclonal antibody (M02), clone 1G8 (Cat # H00026088-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: GGA1 Antibody (1G8) [H00026088-M02] - Detection limit for recombinant GST tagged GGA1 is approximately 0.1ng/ml as a capture antibody.

Applications for GGA1 Antibody (1G8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GGA1

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ADP-ribosylation factor binding protein 1, ADP-ribosylation factor-binding protein GGA1, gamma adaptin ear containing, ARF binding protein 1, gamma-adaptin related protein 1, Gamma-adaptin-related protein 1, golgi-associated, gamma adaptin ear containing, ARF binding protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1

Entrez Gene IDs

26088 (Human)

Gene Symbol

GGA1

OMIM

606004 (Human)

Additional GGA1 Products

Product Documents for GGA1 Antibody (1G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GGA1 Antibody (1G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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