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Ghrelin Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25481

Novus Biologicals, part of Bio-Techne
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NBP3-25481

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Ghrelin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Feline (86%), Canine (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ghrelin Antibody - Azide and BSA Free

Ghrelin Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] -

Immunohistochemistry-Paraffin: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Ghrelin antibody detects secreted Ghrelin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Ghrelin stained by Ghrelin antibody (NBP3-25481) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Ghrelin Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] -

Immunohistochemistry-Paraffin: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Ghrelin antibody detects secreted Ghrelin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Ghrelin stained by Ghrelin antibody (NBP3-25481) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Ghrelin Antibody - Azide and BSA Free

Western Blot: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] -

Western Blot: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Ghrelin antibody (NBP3-25481) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Ghrelin Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Format

Azide and BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ghrelin

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Alternate Names

GHRL, Motilin-related Peptide, Protein M46

Gene Symbol

GHRL

Additional Ghrelin Products

Product Documents for Ghrelin Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ghrelin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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