Ghrelin/Obestatin Antibody (2F4)
Novus Biologicals, part of Bio-Techne | Catalog # H00051738-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2F4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GHRL (AAH25791.1, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDEMEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK
Specificity
GHRL (2F4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Ghrelin/Obestatin Antibody (2F4)
![Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]](https://resources.bio-techne.com/images/products/Ghrelin-Obestatin-Antibody-2F4-Western-Blot-H00051738-M01-img0010.jpg "Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]")
Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]
Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - GHRL monoclonal antibody (M01), clone 2F4. Analysis of GHRL expression in HeLa.![Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]](https://resources.bio-techne.com/images/products/Ghrelin-Obestatin-Antibody-2F4-Immunohistochemistry-Paraffin-H00051738-M01-img0008.jpg "Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]")
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - Analysis of monoclonal antibody to GHRL on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.![ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]](https://resources.bio-techne.com/images/products/Ghrelin-Obestatin-Antibody-2F4-ELISA-H00051738-M01-img0011.jpg "ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]")
ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]
ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.Applications for Ghrelin/Obestatin Antibody (2F4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Ghrelin/Obestatin
Alternate Names
appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46
Entrez Gene IDs
51738 (Human)
Gene Symbol
GHRL
OMIM
601665 (Human)
UniProt
Additional Ghrelin/Obestatin Products
Product Documents for Ghrelin/Obestatin Antibody (2F4)
Product Specific Notices for Ghrelin/Obestatin Antibody (2F4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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