Ghrelin Receptor/GHSR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16655

Novus Biologicals, part of Bio-Techne
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NBP2-16655
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GHSR Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Ghrelin Receptor/GHSR. The exact sequence is proprietary.

Localization

Cell membrane; Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ghrelin Receptor/GHSR Antibody

Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655]

Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655]

Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Ghrelin Receptor/GHSR Antibody [NBP2-16655]

Immunohistochemistry-Paraffin: Ghrelin Receptor/GHSR Antibody [NBP2-16655]

Immunohistochemistry-Paraffin: Ghrelin Receptor/GHSR Antibody [NBP2-16655] - CL1-0 xenograft, using GHS-R1 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655]

Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655]

Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
View 1 more images

Applications for Ghrelin Receptor/GHSR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GHSR

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. [provided by RefSeq]

Long Name

Growth Hormone Secretagogue Receptor

Alternate Names

GH-releasing peptide receptor, Ghrelin receptor, GHRP, GHS-R, growth hormone secretagogue receptor, growth hormone secretagogue receptor type 1

Gene Symbol

GHSR

UniProt

Q92847 (Human)

Additional GHSR Products

Product Documents for Ghrelin Receptor/GHSR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ghrelin Receptor/GHSR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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