GIF Antibody (012)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90226
Recombinant Monoclonal Antibody
![Western Blot: GIF Antibody (012) [NBP2-90226]](https://resources.bio-techne.com/images/products/GIF-Antibody-012-Western-Blot-NBP2-90226-img0004.jpg "Western Blot: GIF Antibody (012) [NBP2-90226]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 012
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for GIF Antibody (012)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human GIF (Uniprot#: P27352-1; Met1-Tyr417).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for GIF Antibody (012)
Western Blot: GIF Antibody (012) [NBP2-90226]
Western Blot: GIF Antibody (012) [NBP2-90226] - Anti-GIF rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.![Immunoprecipitation: GIF Antibody (012) [NBP2-90226]](https://resources.bio-techne.com/images/products/GIF-Antibody-012-Immunoprecipitation-NBP2-90226-img0002.jpg "Immunoprecipitation: GIF Antibody (012) [NBP2-90226]")
Immunoprecipitation: GIF Antibody (012) [NBP2-90226]
Immunoprecipitation: GIF Antibody (012) [NBP2-90226] - GIF was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-GIF rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-GIF rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.![Immunoprecipitation: GIF Antibody (012) [NBP2-90226]](https://resources.bio-techne.com/images/products/GIF-Antibody-012-Immunoprecipitation-NBP2-90226-img0003.jpg "Immunoprecipitation: GIF Antibody (012) [NBP2-90226]")
Immunoprecipitation: GIF Antibody (012) [NBP2-90226]
Immunoprecipitation: GIF Antibody (012) [NBP2-90226] - GIF was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-GIF rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-GIF rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody:Applications for GIF Antibody (012)
Application
Recommended Usage
Immunoprecipitation
0.2-1 ul/mg of lysate
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GIF
Long Name
Gastric Intrinsic Factor
Alternate Names
IF, IFMH, INF, TCN3
Gene Symbol
CBLIF
Additional GIF Products
Product Documents for GIF Antibody (012)
Product Specific Notices for GIF Antibody (012)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...